Journal: STAR Protocols

Article Title: Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

doi: 10.1016/j.xpro.2025.103731

Figure Lengend Snippet:

Article Snippet: ImmPRESS HRP Horse Anti-Mouse IgG Polymer Detection Kit , Vector Laboratories, US , Cat#MP7402.

Techniques: Polymer, Plasmid Preparation, Recombinant, Ex Vivo, Labeling, Enzyme-linked Immunosorbent Assay, Clonogenic Cell Survival Assay, Isolation, Software, Microscopy, Chemiluminescence Immunoassay

Journal: STAR Protocols

Article Title: Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

doi: 10.1016/j.xpro.2025.103731

Figure Lengend Snippet:

Article Snippet: ImmPRESS HRP Horse Anti-Rabbit IgG Polymer Detection Kit , Vector Laboratories, US , Cat#MP-7401.

Techniques: Polymer, Plasmid Preparation, Recombinant, Ex Vivo, Labeling, Enzyme-linked Immunosorbent Assay, Clonogenic Cell Survival Assay, Isolation, Software, Microscopy, Chemiluminescence Immunoassay

Journal: Viruses

Article Title: Two RSV Platforms for G, F, or G+F Proteins VLPs

doi: 10.3390/v12090906

Figure Lengend Snippet: VLP-immunized mice generate serum antibodies against G and/or F. ( A ) Schematic schedule of animal experiments. ( B ) Sera from immunized animals (diluted 1:200) were tested by ELISA for Ga, Gb, or F protein-binding antibodies. After blocking, plates were incubated with goat anti mouse IgG-HRP secondary antibody. OPD substrate was used to develop reaction and absorbance at 490 nm was read. Note the VLPs efficiently induced antibodies against Ga and F but not Gb. ( C ) Sera from immunized animals were heat inactivated at 56 °C for 30 min followed by two-fold serial dilution in triplicate. The dilutions were incubated with 100 TCID 50 of RSV A2 virus for 1h at RT. The mixtures were then transferred to monolayer HEp-2 cell and incubated for 1 h at 37 °C in 5% CO 2 . 5% FBS + MEM media was added to the cells followed by incubation for 72 h at 37 °C in 5% CO 2 . Cells were fixed and ELISA was performed using goat anti-RSV antibody and HPR-conjugated donkey anti-goat secondary antibody. Reaction was developed by OPD and absorbance read at 490 nm. Neutralizing titers were calculated using Reed–Muench method. Note low levels of neutralizing antibodies were induced by the VLPs.

Article Snippet: All HRP-conjugated secondary antibodies used in enzyme-linked immunosorbent assays (EIAs) were obtained from Jackson ImmunoResearch (West Grove, PA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Binding, Blocking Assay, Incubation, Serial Dilution, Virus, Endpoint Dilution Assay